Cell Culture Media

ESF AF Insect Cell Culture Medium, Animal Free

ESF AF™ Insect Cell Culture Medium is an Animal Origin-Free formulation and is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells including Sf9, Sf21, Tni, and Drosophila S2. ESF AF medium is comparable to ESF 921 for growth and expression. ESF AF contains L-Glutamine and Kolliphor® P188 (Pluronic® F-68).


Expression Systems’ ESF AF is an animal origin-free formulation for insect cell culture. It has been thoroughly tested on Sf9, Sf21, Tni (High FiveTM) and S2 cell lines and is comparable to ESF 921 for growth and expression. ESF AF is the logical choice for further manufacturing under cGMP/FDA regulatory compliance and has a Drug Master File with the US FDA.

  • 100% animal component-free
  • Comparable to ESF 921 medium for robust cell growth and expression
  • Sf9 cell densities of 20 x 106 cells/ml or greater    
  • Ideal for scale-up manufacturing
  • Works with a wide variety of baculovirus and host cell platforms
  • Optimized for applications such as production of sub-unit vaccines, virus-like particles and gene therapy vectors (rAAV — all serotypes)
  • Available in 1L bottles
  • Complete, ready-to-use, contains L-glutamine
  • Always in stock for immediate shipment
  • Adapted cells available in suspension
  • Inquire about custom formulations, packaging for bioreactors and master cell bank availability for licensing
Media typeESF AF Insect Cell Culture Medium, Animal Free
Platform compatibilityFunctions with BestBac™, Bacmid, Bac-to-Bac®, BacMam™, MultiBac™ and other BEVS platforms
Cell lines Spodoptera frugiperda (Sf9, Sf21), Trichoplusia ni (Tni and High Five™), and Drosophilia melanogaster (S2)
Shipping conditionAmbient
Storage condition2-8⁰C, protect from light
Use-by dateOne year from date of manufacture

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Bush, M., Setiaputra, D., Yip, C. K., & Molday, R. S. (2016). Cog-Wheel Octameric Structure of RS1, the Discoidin Domain Containing Retinal Protein Associated with X-Linked Retinoschisis. PLoS ONE, 11(1), e0147653. http://doi.org/10.1371/journal.pone.0147653

Dyka, F. M., Wu, W. W. H., Pfeifer, T. A., Molday, L. L., Grigliatti, T. A., & Molday, R. S. (2008). Characterization and Purification of the Discoidin Domain Containing Protein Retinoschisin (RS1) and Its Interaction with Galactose. Biochemistry, 47(35), 9098–9106. http://doi.org/10.1021/bi800938g

Betting, D. J., Mu, X. Y., Kafi, K., McDonnel, D., Rosas, F., Gold, D. P., & Timmerman, J. M. (2009). Enhanced immune stimulation by a therapeutic lymphoma tumor antigen vaccine produced in insect cells involves mannose receptor targeting to antigen presenting cells. Vaccine, 27(2), 250–259. http://doi.org/10.1016/j.vaccine.2008.10.055

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99-300-01ESF AF Insect Cell Culture Medium, Animal Free 1L Bottle2–8º CAmbient$52.00