Services

Your Contract Manufacturing Organization

Leveraging its experience, expertise, and passion for the BEVS platform, Expression Systems provides a comprehensive array of services for manufacturing recombinant proteins, vaccines and gene therapy vectors. We specialize in the BEVS platform and take pride in our history of successfully completing projects where other more generalized CMOs have failed. For contract manufacturing from research-grade to GMP production applications, we have the expertise and manufacturing capabilities to develop or produce your products from start to finish or “a la carte.”

Biotech firms, research institutions, and consultants need a Contract Manufacturing Organization (CMO) they can trust — an organization that has the scientific expertise and manufacturing capabilities to develop or produce a current Good Manufacturing Practice-compliant product successfully, on budget and on time.

Expression System’s capable team provides superb customer service and communication, expert process development, high-quality products at an outstanding value, and manufacturing process ownership. We invite you to visit and meet the team, review capabilities & quality systems, and tour our outstanding new facility, designed specifically for phase 1 and 2 production.

Expression Systems’ contract production services can assist you at any step of the process, solving technical problems in molecular biology, purification or other issues relating to BEVS.

Molecular Biology

Expression Systems can clone your gene of interest into any commercial baculovirus expression vector or our own vectors. Starting from your cDNA clone or an accession number we will guide expression cassette design and clone your gene into a transfer vector. We can add tags at the N or C terminus, add secretion signals, and do site-directed mutagenesis. We will sequence validate the expression vector for you and produce recombinant baculovirus from your clone.

Molecular biology services include

Gene sequence codon optimization
Gene synthesis
Vector design and cloning
Plasmid scale up and purification

Baculovirus Stock Production

Expression Systems can quickly produce baculovirus stock production from your gene of interest. The final product can be a minimum of 500 ml up to hundreds of liters at a titer of at least 5 x 108 infectious units per ml. Titer is determined using the flow cytometric based assay that is also offered as a service. The stock can be generated from a pre-existing virus stock or by cotransfection using a transfer vector containing the gene of interest provided by you. Baculovirus stocks are generated using Sf9 cells in your choice of ESF 921 or ESF AF media. If desired, baculovirus stocks can be purified by end point dilution or plaque purification.

Baculovirus stock production services include

Production of high titer (> 5 x 108) baculovirus stock
Virus stock scale up to 100s of liters
Baculovirus stock purification by end point dilution or plaque purification
Titering services

Protein Production

Expression Systems will optimize the protein production parameters for your product. Recombinant protein, virus-like particle or ene therapy vector expression will be optimized in one or more cell lines by doing a time-course study of infection, varying infection density, multiplicity of infection (MOI), and harvest time. Scale up conditions for protein production and process development are also offered by Expression Systems.
Expression Systems has dedicated clean rooms for your protein production project. Inquire about GMP/GLP manufacturing. Expression Systems will produce your protein of interest using conditions specified by you or determined by the expression optimization.