ESF 921 Delta Series
ESF 921 Delta Series Formulations are based on the popular ESF 921 medium and are available in a variety of amino acid deficient formats used for structural analysis and protein labeling. Off the shelf formulations include all amino acid deficient and methionine deficient. Select amino acid deficiencies may be custom ordered.
Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion. To support the efforts of scientists using this methodology, Expression Systems supplies a Methionine Deficient medium formulation. Se-Met incorporation levels are reported to be typically 75% (Protein Science 16(9):2023-9 · October 2007) but can range from 70-100% using this medium.
Structural biologists are increasingly utilizing NMR spectroscopy to obtain information about the structure and dynamics of proteins and their complexes. The simplest and most cost-effective biosynthetic labeling method for protein solid-state NMR is to uniformly label all carbon and nitrogen atoms with 13C and 15N. Expression Systems stocks an all amino acid deficient variant within the Delta series to enable scientists interested in adding a source of isotopically-labeled amino acids. Custom formulations may also be requested specifying a formulation deficient in carbon sources or specific amino acids. Custom formulations require a 20L minimum order and are delivered in approximately 6 weeks.
|Media type||Serum-free liquid, 1X|
|Platform compatibility||Functions with BestBac™, Bacmid, Bac-to-Bac®, BacMam™, MultiBac™ and other BEVS platforms|
|Cell lines||Spodoptera frugiperda (Sf9, Sf21), Trichoplusia ni (Tni and High Five™), and Drosophilia melanogaster (S2)|
|Storage condition||2-8⁰C, protect from light|
|Use-by date||One year from date of manufacture|
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Production of selenomethionyl-derivatized proteins in baculovirus-infected insect cells
Ciarán N. Cronin, Kheng B. Lim, Joe Rogers
Protein Sci. 2007 Sep; 16(9): 2023–2029. doi: 10.1110/ps.072931407