Baculovirus Titering Service
Expression Systems is pleased to offer baculovirus titering services to help our customers maximize expression and reproducibility. Accurate titering of recombinant baculovirus is an important step in protein production optimization. A high throughput flow cytometric assay is utilized to quantitate infectious baculovirus particles produced in insect cell culture, allowing for accurate virus titer determination in less than 24 hours. The assay detects the cell surface expression of gp64 after a short incubation allowing for a single round of infection. Virus-infected cells are then assessed by flow cytometric analysis for gp64 expression.
Baculovirus titering is routinely set up on Wednesdays with data delivery Thursday afternoon PST. Samples should be received by noon Wednesday to be set up that week.
How much do I need to ship?
We prefer 1.0 mL but can run the assay with 0.5 mL. If necessary, we can run the assay with as little 200 μL, but this requires advance notice.
How do I need to ship my virus samples?
Samples must be sterile and should ideally be packaged in cryovials or other small screw cap tubes. Eppendorf tubes and 15 mL conical tubes are not appropriate. We have experienced cracking of 15 mL conical tubes during shipment.
Samples should NOT be frozen, but should be shipped overnight on cold packs. For customers that would like to submit more than 10 samples, we request advance notice (for instance the Monday before they ship).
How do I place an order?
To place a phone order, complete the titering service form and phone in payment by calling 530 747 2035. To pay by purchase order, credit card or debit card, visit the Baculovirus Titering Service page
Send your titering samples on cold pack to:
Attention: Titering Service
2537 2nd Street
Davis, CA 95618
Titer data will be emailed to you Thursday afternoon PST for samples received by noon on Wednesday each week. If you would like a price quote, please email firstname.lastname@example.org.