pVL1392, pVL1393 Transfer Vectors
pVL1392 and pVL1393 are transfer vectors designed for high-level expression of a cloned gene driven by the strong Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedrin promoter (pPolh).
pVL1392 and pVL1393 are pUC8 based plasmids containing the AcMNPV polyhedrin locus lacking the majority of the polyhedrin open reading frame. A multiple cloning site (MCS) sequence has been inserted 37 nucleotides downstream from the original polyhedrin ATG start codon, which has been mutated to ATT.
pVL1392 and pVL1393 share identical sequences, with the exception that they each have the same MCS in opposing orientations. A start and stop codon must be provided with the gene that is to be cloned into the MCS. For maximal recombinant protein expression, the distance between the cloning site and the ATG start of the inserted gene should be kept to a minimum, and should not exceed 100 nucleotides.
|Media Type||ESF 921 and ESF AF|
|Platform Compatibility||BestBac 1.0 and BestBac 2.0|
|Storage condition||2-8°C, protect from light|