Baculovirus Titering Service
Expression Systems is pleased to offer baculovirus titering service to help our customers maximize expression and reproducibility. Baculovirus titering is routinely set up on Wednesdays with data delivery Thursday afternoon PST. Samples should be received by 11AM Wednesday to be set up that week.
Accurate titering of recombinant baculovirus is an important step in protein production optimization. A high throughput, flow cytometric assay is utilized to quantitate infectious baculovirus particles produced in insect cell culture, allowing for accurate virus titer determination for multiple conditions in less than 24 hours. The assay detects the cell surface expression of gp64 after a short incubation period allowing for a single round of infection. Virus infected cells are then assessed by flow cytometric analysis for gp64 expression.
Ordering and Shipping Instructions
It’s an easy process to order titering services from Expression Systems:
- Complete the Titering Service Form attached here and the Sample Submission Form attached here.
- Print a copy of the completed form to be included with your shipped samples.
- Email a copy of the completed form to titering@expressionsystems.com
. - Update the “Sample Quantity” below to match the number of samples you are shipping, click the “Proceed to Checkout” button and complete the checkout process
$195.00
Baculovirus Titering Service : $195.00/Sample
Shipping
- Ship 0.5-1 ml of virus stock (as little as 200 µl can be titered with advance notice)
- Ship sterile aliquots in 2 ml screw cap tubes or equivalent. Ship overnight on cold packs.
Ship to:
Attention: Titering Service Expression Systems 2537 2nd Street Davis, CA 95618 Phone: 530-747-2035
Baculovirus Titer Service FAQ
How much virus do I need to ship?
We prefer 1.0 mL but can run the assay with 0.5 mL. If necessary, we can use as little 200 μL but that requires advance notice.
How do I need to ship my virus samples?
Samples need to be sterile and should be ideally sent in cryovials or some other small screw cap tube. Eppendorf tubes and 15 mL conicals are not appropriate. (We have experienced cracking of 15 mL conical tubes during shipment.)
The sample should NOT be frozen but should be shipped overnight on cold packs. For customers that would like to submit more than 10 samples, we request advance notice (the Monday before they ship, for example, is a reasonable notice).
How do I place an order?
To place a phone order, complete the titering service form and phone in payment by calling 530 747 2035. To pay by purchase order, credit card or debit card, visit the Baculovirus Titering Service page
Where do I ship my samples?
Attention: Titering Service Expression Systems 2537 2nd Street Davis, CA 95618 Phone: 530-747-2035
When will I receive my results?
Titer data for samples received by noon on Wed are emailed Thursday afternoon Pacific Standard Time.
Mulvania T, Hayes B, Hedin D. A Flow Cytometric Assay for Rapid, Accurate Determination of Baculovirus Titers. BioProcess J, 2004; 3(3): 47-53.