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string(585) "Expression Systems strongly supports a rigorous observation program of cell cultures rather than relying on a finite number of passages. Culture conditions such as temperature fluctuations, over-growing, under-seeding, oxygen deprivation and other environmental stresses play a far greater role than a passage count. A good rule of thumb is no more than 30 passages as this limits the number of environmental insults the culture has to withstand. Subtle morphological changes in the culture such as cell swelling, elongation or increased clumping are signals to initiate a new culture."
["post_title"]=>
string(39) "What is the passage limit for my cells?"
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Expression Systems strongly supports a rigorous observation program of cell cultures rather than relying on a finite number of passages. Culture conditions such as temperature fluctuations, over-growing, under-seeding, oxygen deprivation and other environmental stresses play a far greater role than a passage count. A good rule of thumb is no more than 30 passages as this limits the number of environmental insults the culture has to withstand. Subtle morphological changes in the culture such as cell swelling, elongation or increased clumping are signals to initiate a new culture.
