Reagents

gp64-PE (R-PHYCOERYTHRIN (R-PE)-CONJUGATED MOUSE ANTI-BACULOVIRUS MONOCLONAL ANTIBODY)

Expression Systems offers a PE-labeled antibody directed to gp64, the baculovirus envelope protein required for cell-to-cell spread.

Description

The baculovirus gp64 envelope glycoprotein is a major component of the envelope of the budded baculovirus and is essential for entry into cultured insect host cells. The anti-gp64 antibody is effective for direct immunofluorescence staining of insect cells for flow cytometric analysis. Applications include baculovirus titration, infection kinetics monitoring and protein expression screening.

Contents:

gp64-PE (R-PHYCOERYTHRIN (R-PE)-CONJUGATED MOUSE ANTI-BACULOVIRUS MONOCLONAL ANTIBODY)

Clone: AcV1

Antibody Composition: Mouse IgG2A

Storage Buffer: Phosphate buffered saline (PBS) containing 0.05% BSA

and 0.09% sodium azide.

Specification
Media TypeESF 921 or ESF AF
Platform compatibilityWorks with infectious baculovirus produced by most methods
Shipping conditionAmbient
Storage condition2-8°C, protect from light
Use-by Date2 years from Date of Manufacture
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References

Hohmann AW, Faulkner P. Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis. Virology. 1983 Mar;125(2):432-44.
Mulvania T, Hayes B, Hedin D. A Flow Cytometric Assay for Rapid, Accurate Determination of Baculovirus Titers. BioProcess J, 2004; 3(3): 47-53.

Zhou J, Blissard GW. Mapping the conformational epitope of a neutralizing antibody (AcV1) directed against the AcMNPV GP64 protein. Virology. 2006 Sep 1;352(2):427-37.

Kumar M, Bradow BP, Zimmerberg J. Large-scale production of pseudotyped lentiviral vectors using baculovirus GP64. Hum Gene Ther. 2003 Jan 1;14(1):67-77.

Volkman LE, Goldsmith PA. Resistance of the 64K protein of budded Autographa californica nuclear polyhedrosis virus to functional inactivation by proteolysis. Virology. 1988 Sep;166(1):285-9.

ItemDescriptionRecommended
Storage Condition
Shipping
Condition
PriceQTY
97-201gp64-PE (R-PHYCOERYTHRIN (R-PE)-CONJUGATED MOUSE ANTI-BACULOVIRUS MONOCLONAL ANTIBODY) 200ul2–8º CAmbient$300.00