Reagents

Production Boost Additive

Expression Systems’ Production Boost Additive (PBA) is a nutrient boost designed to increase protein expression by supporting the increased needs of insect cells infected by recombinant baculovirus. PBA is a serum-free, animal-free formulation that complements ESF 921 and ESF AF insect cell culture media and extends the late and very-late expression period of production by maintaining high culture viability.

Description

Expression Systems’ Production Boost Additive (PBA) is a nutrient
boost designed to increase protein expression by supporting the
increased needs of insect cells infected by recombinant baculovirus.
PBA is a serum-free, animal-free formulation that complements ESF 921
and ESF AF insect cell culture media and extends the late and very-late
expression period of production by maintaining high culture viability.
PBA is a cocktail of nutrients designed for supplementation at a rate of
1–10% at 6–36 hours post infection.

  • Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
  • Extends culture period by maintaining cell viability
  • Increases protein expression                
  • Available in 100 mL or 1 L bottles—inquire for custom packaging
  • In stock for immediate shipment

Fig. 1          Fig. 2

Tni Pro cells (Fig. 1) were infected with a recombinant baculovirus at an MOI of 1. PBA was added at 1% of the culture volume 18 hours post infection. Sf9 cells (Fig. 2) were infected at an MOI of 0.02. PBA was added at 1% or 5% of the culture volume 36 hours post infection. The recombinant protein was secreted into the culture supernatant and harvested daily. Protein expression levels were determined by protein specific ELISA.

Specification
Media typeSerum-free, animal-free liquid, 1X
Shipping conditionAmbient
Storage condition2–8⁰C, protect from light
Use-by dateOne year from date of manufacture
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Enter Lot # for product
ItemDescriptionRecommended
Storage Condition
Shipping
Condition
PriceQTY
95-006-01Production Boost Additive1L Bottle2–8º CAmbient$525.00
95-006-100Production Boost Additive100ml2–8º CAmbient$65.00