Cells

Insect Cells

Expression Systems offers insect cell lines adapted to serum-free suspension culture in either ESF 921 or ESF AF. The availability of pre-adapted cells saves significant time and expense associated with the adaptation of cultures.

Description

Sf9 Insect Cells

The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE.  Sf9 cells are adapted for suspension culture in ESF 921 or ESF AF and are available as a frozen vial or suspension culture.Sf9 cells are commonly used to produce recombinant baculoviral stocks and to produce recombinant proteins. 
• Recombinant protein expression from a variety of expression platforms
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Quality and performance testing

Sf21 Insect Cells

The Sf21 insect cell line (IPLB-Sf-21-AE) is derived from ovarian cells of Spodoptera frugiperda.  Sf21 cells are adapted for suspension culture in ESF 921 or ESF AF and are available as a frozen vial or suspension culture.

• Recombinant protein expression from a variety of expression platforms
• Good growth in adherent or suspension culture
• Quality and performance testing

Tni Insect Cells

The Tni insect cell line is derived from ovarian cells isolated from Trichoplusia ni.  Tni cells are adapted for suspension culture in ESF 921 or ESF AF and are available as a frozen vial or suspension culture.

• Recombinant protein expression from a variety of expression platforms
• Can express 5-10x as much protein as Spodoptera cell lines
• Good growth in adherent or suspension culture
• Quality and performance testing

S2 Insect Cells

The S2 insect cell line is derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos. S2 cells are adapted for suspension culture in ESF 921 or ESF AF and are available as a frozen vial or suspension culture.

• Recombinant protein expression from transient expression or stable cell line
• Good growth in adherent or suspension culture
• Quality and performance testing

Specification
Media TypeESF 921 or ESF AF
Shipping conditionAmbient for suspension cultures, dry ice for frozen vials
Storage conditionCulture suspension shipments immediately, liquid nitrogen (vapor phase) for frozen vials.
Safety InformationTreat as Biosafety Level 1
Documents
ItemDescriptionRecommended
Storage Condition
Shipping
Condition
PriceQTY
94-001FSf9 cellsFrozen in ESF 92150M cells/vialLN²Dry ice$250.00
94-001SSf9 cellsSuspension in ESF 9211M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00
94-002FTni cellsFrozen in ESF 92150M cells/vialLN²Dry ice$250.00
94-002STni cellsSuspension in ESF 9211M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00
94-003FSf21 cellsFrozen in ESF 92150M cells/vialLN²Dry ice$250.00
94-003SSf21 cellsSuspension in ESF 9211M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00
94-005FS2 Drosophila cellsFrozen in ESF 921100M cells/vialLN²Dry ice$250.00
94-005SS2 Drosophila cellsSuspension in ESF 9211M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00
94-006FSf9 cellsFrozen in ESF AF50M cells/vialLN²Dry ice$250.00
94-006SSf9 cellsSuspension in ESF AF1M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00
94-010FSf21 cellsFrozen in ESF AF50M cells/vialLN²Dry ice$250.00
94-010SSf21 cellsSuspension in ESF AF1M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00
94-011FTni cellsFrozen in ESF AF50M cells/vialLN²Dry ice$250.00
94-011STni cellsSuspension in ESF AF1M cells/mL (50mL/125mL flask)Culture upon receiptAmbient$150.00